科學家繪製人類胸腺細胞圖譜
作者：
小柯機器人發布時間：2020/2/22 10:55:25
英國威康桑格研究所Sarah A. Teichmann、Muzlifah Haniffa和比利時根特大學Tom Taghon課題組合作提出人類胸腺發育的細胞圖譜定義T細胞庫的形成。該項研究成果發表在2020年2月21日出版的《科學》雜誌上。
他們使用單細胞RNA測序在整個人類生命周期內進行胸腺細胞普查，並重建T細胞分化軌跡和T細胞受體（TCR）重組動力學。使用這種方法，他們確定並定位了原位CD8αα+ T細胞群體、胸腺成纖維細胞亞型和激活的樹突狀細胞狀態。此外，他們揭示了TCR重組和選擇的偏好，這歸因於基因組位置和譜系定型動力學。綜上所述，他們的數據提供了人類整個生命周期胸腺綜合圖譜，並對人類T細胞發育提供了新的見解。
研究人員表示，胸腺為T細胞的分化和選擇提供了一個滋養環境，這一過程由多種類型胸腺細胞的相互作用所協調。
附：英文原文
Title: A cell atlas of human thymic development defines T cell repertoire formation
Author: Jong-Eun Park, Rachel A. Botting, Cecilia Domínguez Conde, Dorin-Mirel Popescu, Marieke Lavaert, Daniel J. Kunz, Issac Goh, Emily Stephenson, Roberta Ragazzini, Elizabeth Tuck, Anna Wilbrey-Clark, Kenny Roberts, Veronika R. Kedlian, John R. Ferdinand, Xiaoling He, Simone Webb, Daniel Maunder, Niels Vandamme, Krishnaa T. Mahbubani, Krzysztof Polanski, Lira Mamanova, Liam Bolt, David Crossland, Fabrizio de Rita, Andrew Fuller, Andrew Filby, Gary Reynolds, David Dixon, Kourosh Saeb-Parsy, Steven Lisgo, Deborah Henderson, Roser Vento-Tormo, Omer A. Bayraktar, Roger A. Barker, Kerstin B. Meyer, Yvan Saeys, Paola Bonfanti, Sam Behjati, Menna R. Clatworthy, Tom Taghon, Muzlifah Haniffa, Sarah A. Teichmann
Issue&Volume: 2020/02/21
Abstract: AbstractThe thymus provides a nurturing environment for the differentiation and selection of T cells, a process orchestrated by their interaction with multiple thymic cell types. We used single-cell RNA sequencing to create a cell census of the human thymus across the life span and to reconstruct T cell differentiation trajectories and T cell receptor (TCR) recombination kinetics. Using this approach, we identified and located in situ CD8αα+ T cell populations, thymic fibroblast subtypes, and activated dendritic cell states. In addition, we reveal a bias in TCR recombination and selection, which is attributed to genomic position and the kinetics of lineage commitment. Taken together, our data provide a comprehensive atlas of the human thymus across the life span with new insights into human T cell development.
DOI: 10.1126/science.aay3224
Source: https://science.sciencemag.org/content/367/6480/eaay3224
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