COVID-19患者的有效中和抗體揭示多個靶標弱點
作者：
小柯機器人發布時間：2020/6/17 20:43:39
荷蘭阿姆斯特丹大學Marit J. van Gils、Rogier W. Sanders、Godelieve J. de Bree等研究人員合作發現，COVID-19患者的有效中和抗體揭示了多個靶標弱點。該研究於2020年6月15日在線發表在《科學》雜誌上。
研究人員使用SARS-CoV-2穩定融合前刺突蛋白從三名恢復期的COVID-19患者中分離了單克隆抗體。這些抗體的體細胞超突變水平較低，並且在VH1-69、VH3-30-3和VH1-24基因使用中顯示出很強的富集性。一類抗體能夠在低至0.007μg/ mL時有效抑制真實SARS-CoV-2感染。
競爭和電子顯微鏡研究表明，SARS-CoV-2突刺蛋白包含了多個不同的抗原位點，包括幾個受體結合域（RBD）表位以及非RBD表位。因此，除了為疫苗設計提供指導之外，這些抗體也是COVID-19治療和預防的潛在候選。
附：英文原文
Title: Potent neutralizing antibodies from COVID-19 patients define multiple targets of vulnerability
Author: Philip J. M. Brouwer, Tom G. Caniels, Karlijn van der Straten, Jonne L. Snitselaar, Yoann Aldon, Sandhya Bangaru, Jonathan L. Torres, Nisreen M. A. Okba, Mathieu Claireaux, Gius Kerster, Arthur E. H. Bentlage, Marlies M. van Haaren, Denise Guerra, Judith A. Burger, Edith E. Schermer, Kirsten D. Verheul, Niels van der Velde, Alex van der Kooi, Jelle van Schooten, Marille J. van Breemen, Tom P. L. Bijl, Kwinten Sliepen, Aafke Aartse, Ronald Derking, Ilja Bontjer, Neeltje A. Kootstra, W. Joost Wiersinga, Gestur Vidarsson, Bart L. Haagmans, Andrew B. Ward, Godelieve J. de Bree, Rogier W. Sanders, Marit J. van Gils
Issue&Volume: 2020/06/15
Abstract: Abstract The rapid spread of SARS-CoV-2 has a significant impact on global health, travel and economy. Therefore, preventative and therapeutic measures are urgently needed. Here, we isolated monoclonal antibodies from three convalescent COVID-19 patients using a SARS-CoV-2 stabilized prefusion spike protein. These antibodies had low levels of somatic hypermutation and showed a strong enrichment in VH1-69, VH3-30-3 and VH1-24 gene usage. A subset of the antibodies were able to potently inhibit authentic SARS-CoV-2 infection as low as 0.007 μg/mL. Competition and electron microscopy studies illustrate that the SARS-CoV-2 spike protein contains multiple distinct antigenic sites, including several receptor-binding domain (RBD) epitopes as well as non-RBD epitopes. In addition to providing guidance for vaccine design, the antibodies described here are promising candidates for COVID-19 treatment and prevention.
DOI: 10.1126/science.abc5902
Source: https://science.sciencemag.org/content/early/2020/06/15/science.abc5902
Science：《科學》，創刊於1880年。隸屬於美國科學促進會，最新IF：41.037