以下是 2019 年圖像大賽的得獎名單與圖像，非常感謝各位參加者的作品，也非常感謝各位於2019年的支持。
祝各位聖誕節快樂!
1st Prize WinnerImage from Howard Vindin, PhD candidate, Weiss Lab, Charles Perkins Centre, The University of Sydney, NSW, AustraliaThis single channel 350GB image shows Erythrocytes that mark the developing vasculature in a mouse embryo (E14.5). The 8x11x2mm specimen was imaged on a multiphoton microscope excited at 790nm using a 25x 1.0NA Objective. Subsequently, the image was stitched, deconvolved with Huygens Professional using a measured PSF, and visualised with depth-coded colouring using the Huygens MIP renderer (version 19.10). Howard's instagram account (#artofmicroscopy) shows many great images and nearly all of them have been processed with the help of Huygens .
2nd Prize Winner Dr. Marta Cortes Canteli, Miguel Servet Research Fellow, Centro Nacional de Investigaciones Cardiovasculares Carlos III, Spain.This Huygens deconvolved image shows hypertrophic and reactive astrocytes (GFAP-green) present in the brain parenchyma of transgenic Alzheimer’s disease mice. The confocal z-stack was acquired according to Nyquist criteria using a HC PL APO 93X/1.30 GLYC motCORR objective of a Leica TCS SP8 Confocal-gSTED 3D System. The data was deconvolved with Huygens Professional. Finally, a 3D surface was obtained using the SFP Volume Renderer of Huygens Professional Software. Isosurface of the green channel was applied as indicated.
3th Prize Winner
Mrs. Karin Panser, Pauli Group, Institute of Molecular Pathology, Austria.
This Huygens deconvolved image shows an optical section through somites of a 2-day old zebrafish tail, stained for actin (phalloidin, shown in orange-gold) and nuclei (DAPI, shown in blue). The image was acquired on a Zeiss LSM700 confocal (40x, 1.3 NA) and deconvolved with Huygens Professional.
4th Prize Winner
Dr. Grazvydas Lukinavicius, MPI for Biophysical Chemistry - Department of NanoBiophotonics, Max Planck Institute, Germany.
This Huygens deconvolved image shows a IsoSTED image of tubulin labelled with a small molecule probe, and imaged on an Abberior STED expert line microscope. Confocal and 3D STED images were deconvolved using Huygens software. Movie generated using Huygens MIP renderer.
5th Prize Winner
Dr. Ioannis Alexopoulos (General Instrumentation), sample provided by Dr. Ivo Rieu & Prof. Dr. Jian Xu and prepared by Mieke Wolters (Molecular Plant Physiology), Faculty of Science, Radboud University, Netherlands.
Multiview light sheet data (3 angles: -135deg, 45deg, 135 degrees) was fused and deconvolved with Huygens FUSER, and shows Arabidopsis thaliana mature anther with pollen grains, stained with Alexander's staining protocol. Multiview images were taken with an Olympus/PhaseView Alpha3 Light Sheet system. Image was visualized with the Huygens MIP Renderer using depth-code colouring.