腸道微生物群為肝硬化診斷提供便利
作者：
小柯機器人發布時間：2020/7/1 20:36:25
美國加州大學Rohit Loomba和索爾克生物研究所Ronald M. Evans研究組合作取得進展。他們揭示了可以預測肝硬化的通用腸道微生物組特徵。這一研究成果於2020年6月30日在線發表在《細胞-代謝》上。
為了確定肝硬化診斷與腸道微生物的相關程度，研究人員比較了包括非-非酒精性脂肪性肝（NAFLD）對照、NAFLD肝硬化患者及其直系親屬在內的163名特徵明確參與者的糞便微生物組。通過使用隨機森林機器學習算法結合鳥槍法宏基因組學和非目標代謝組學譜的差異豐度分析，研究人員發現離散的宏基因組學和代謝組學特徵在檢測肝硬化方面同樣有效（診斷準確度為0.91，曲線下面積[AUC]）。
將宏基因組學特徵與年齡和血清白蛋白水平相結合，可以準確地區分在地理上分離區域病因和遺傳上不同人群的肝硬化。肝硬化患者的血清天冬氨酸轉氨酶水平增加，這可以區分肝硬化和早期纖維化。這些發現表明腸道微生物組種類可能為肝硬化的非侵入性診斷提供便利。
據悉，腸道微生物組紊亂與NAFLD向晚期纖維化和肝硬化的發展有關。
附：英文原文
Title: A Universal Gut-Microbiome-Derived Signature Predicts Cirrhosis
Author: Tae Gyu Oh, Susy M. Kim, Cyrielle Caussy, Ting Fu, Jian Guo, Shirin Bassirian, Seema Singh, Egbert V. Madamba, Ricki Bettencourt, Lisa Richards, Manuela Raffatellu, Pieter C. Dorrestein, Ruth T. Yu, Annette R. Atkins, Tao Huan, David A. Brenner, Claude B. Sirlin, Rob Knight, Michael Downes, Ronald M. Evans, Rohit Loomba
Issue&Volume: 2020-06-30
Abstract: Dysregulation of the gut microbiome has been implicated in the progression of non-alcoholicfatty liver disease (NAFLD) to advanced fibrosis and cirrhosis. To determine the diagnosticcapacity of this association, we compared stool microbiomes across 163 well-characterizedparticipants encompassing non-NAFLD controls, NAFLD-cirrhosis patients, and theirfirst-degree relatives. Interrogation of shotgun metagenomic and untargeted metabolomicprofiles by using the random forest machine learning algorithm and differential abundanceanalysis identified discrete metagenomic and metabolomic signatures that were similarlyeffective in detecting cirrhosis (diagnostic accuracy 0.91, area under curve [AUC]).Combining the metagenomic signature with age and serum albumin levels accurately distinguishedcirrhosis in etiologically and genetically distinct cohorts from geographically separatedregions. Additional inclusion of serum aspartate aminotransferase levels, which areincreased in cirrhosis patients, enabled discrimination of cirrhosis from earlierstages of fibrosis. These findings demonstrate that a core set of gut microbiome speciesmight offer universal utility as a non-invasive diagnostic test for cirrhosis.
DOI: 10.1016/j.cmet.2020.06.005
Source: https://www.cell.com/cell-metabolism/fulltext/S1550-4131(20)30306-5