科學家揭示人組織遷移CD8+ T細胞的特徵

科學家揭示人組織遷移CD8+ T細胞的特徵

作者：

小柯機器人

發布時間：2020/12/12 20:14:43

美國賓夕法尼亞大學Michael R. Betts及瑞典卡羅林斯卡醫學院Marcus Buggert小組合作鑑定了人組織遷移CD8⁺ T細胞的特徵。2020年12月10日，國際學術期刊《細胞》在線發表了這一成果。

為了更深入理解淋巴細胞遷移的功能，研究人員使用了一種集成方法來表徵胸導管淋巴瘤（TDL）中組織遷移細胞的譜系特徵。人和非人靈長類傳導淋巴液中最普遍的免疫細胞是T細胞。具有效應子樣表觀遺傳和轉錄特徵的細胞溶解性CD8⁺ T細胞亞群表現出克隆型傾向並選擇性地局限於血管內循環，而具有莖樣表觀遺傳和轉錄特徵的非溶解性CD8⁺ T細胞亞群在組織和TDL中佔據主導地位。

而且，這些在解剖學上具有不同基因表達譜的亞群在單個克隆型中得以表徵，表明平行分化程序獨立於所表達的抗原受體。該研究數據集提供了免疫細胞遷移的系統圖集，並揭示了通過TDL循環進行組織遷移CD8⁺ T細胞的性質。

據悉，淋巴細胞遷移對於適應性免疫發揮功能至關重要。但是，由於大多數對人淋巴細胞遷移的研究僅限於對血液和各種組織進行免疫分析，因此，目前對這一過程的了解還十分有限。

附：英文原文

Title: The Identity of Human Tissue-Emigrant CD8+ T Cells

Author: Marcus Buggert, Laura A. Vella, Son Nguyen, Vincent H. Wu, Zeyu Chen, Takuya Sekine, André Perez-Potti, Colby R. Maldini, Sasikanth Manne, Samuel Darko, Amy Ransier, Leticia Kuri-Cervantes, Alberto Sada Japp, Irene Bukh Brody, Martin A. Ivarsson, Jean-Baptiste Gorin, Olga Rivera-Ballesteros, Laura Hertwig, Jack P. Antel, Matthew E. Johnson, Afam Okoye, Louis Picker, Golnaz Vahedi, Ernesto Sparrelid, Sian Llewellyn-Lacey, Emma Gostick, Johan K. Sandberg, Niklas Bjrkstrm, Amit Bar-Or, Yoav Dori, Ali Naji, David H. Canaday, Terri M. Laufer, Andrew D. Wells, David A. Price, Ian Frank, Daniel C. Douek, E. John Wherry, Maxim G. Itkin, Michael R. Betts

Issue&Volume: 2020-12-10

Abstract: Lymphocyte migration is essential for adaptive immune surveillance. However, our currentunderstanding of this process is rudimentary, because most human studies have beenrestricted to immunological analyses of blood and various tissues. To address thisknowledge gap, we used an integrated approach to characterize tissue-emigrant lineagesin thoracic duct lymph (TDL). The most prevalent immune cells in human and non-humanprimate efferent lymph were T cells. Cytolytic CD8+ T cell subsets with effector-like epigenetic and transcriptional signatures wereclonotypically skewed and selectively confined to the intravascular circulation, whereasnon-cytolytic CD8+ T cell subsets with stem-like epigenetic and transcriptional signatures predominatedin tissues and TDL. Moreover, these anatomically distinct gene expression profileswere recapitulated within individual clonotypes, suggesting parallel differentiationprograms independent of the expressed antigen receptor. Our collective dataset providesan atlas of the migratory immune system and defines the nature of tissue-emigrantCD8+ T cells that recirculate via TDL.

DOI: 10.1016/j.cell.2020.11.019

Source: https://www.cell.com/cell/fulltext/S0092-8674(20)31536-1

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