單細胞轉錄組學揭示肝實質和非實質細胞譜系的早期出現
作者：
小柯機器人發布時間：2020/10/31 20:57:43
加拿大特裡福克斯實驗室Pamela A. Hoodless團隊近日取得一項新成果。他們揭示單細胞轉錄組學揭示了肝實質和非實質細胞譜系的早期出現。2020年10月29日，《細胞》雜誌發表了這一成果。
他們分析了從胚胎（E）7.5（指定內胚層祖細胞）到E10.5肝（當肝實質和非實質細胞譜系出現時）的45,334個單細胞轉錄組。他們的數據詳細說明了血管和竇狀內皮的差異，包括E8.75對竇狀內皮特異性不同轉的錄譜。
他們表徵兩種不同的間皮細胞類型以及早期肝星狀細胞，並揭示這些人群的獨特時空分布。他們捕獲成肝細胞規範和遷移的轉錄譜，包括肝間充質細胞類型的出現和成肝細胞集體細胞遷移的證據。
此外，他們在原始正弦波的組織過程中確定了細胞間的相互作用。這項研究提供了從內胚層和中胚層一直到原始正弦波組織的單細胞解析度肝臟譜系建立的綜合圖集。
據悉，早期肝臟的細胞複雜性和規模已限制了檢查其在器官發生過程中出現的分析。
附：英文原文
Title: Single-Cell Transcriptomics Reveals Early Emergence of Liver Parenchymal and Non-parenchymal Cell Lineages
Author: Jeremy Lotto, Sibyl Drissler, Rebecca Cullum, Wei Wei, Manu Setty, Erin M. Bell, Stéphane C. Boutet, Sonja Nowotschin, Ying-Yi Kuo, Vidur Garg, Dana Pe』er, Deanna M. Church, Anna-Katerina Hadjantonakis, Pamela A. Hoodless
Issue&Volume: 2020/10/29
Abstract: The cellular complexity and scale of the early liver have constrained analyses examiningits emergence during organogenesis. To circumvent these issues, we analyzed 45,334single-cell transcriptomes from embryonic day (E)7.5, when endoderm progenitors arespecified, to E10.5 liver, when liver parenchymal and non-parenchymal cell lineagesemerge. Our data detail divergence of vascular and sinusoidal endothelia, includinga distinct transcriptional profile for sinusoidal endothelial specification by E8.75.We characterize two distinct mesothelial cell types as well as early hepatic stellatecells and reveal distinct spatiotemporal distributions for these populations. We capturetranscriptional profiles for hepatoblast specification and migration, including theemergence of a hepatomesenchymal cell type and evidence for hepatoblast collectivecell migration. Further, we identify cell-cell interactions during the organizationof the primitive sinusoid. This study provides a comprehensive atlas of liver lineageestablishment from the endoderm and mesoderm through to the organization of the primitivesinusoid at single-cell resolution.
DOI: 10.1016/j.cell.2020.09.012
Source: https://www.cell.com/cell/fulltext/S0092-8674(20)31153-3
Cell：《細胞》，創刊於1974年。隸屬於細胞出版社，最新IF：36.216