中國科學家揭示囊泡介導非常規蛋白分泌的轉運途徑
作者：
小柯機器人發布時間：2020/4/14 13:43:40
近日，清華大學葛亮課題組報導了一條囊泡介導非常規蛋白分泌的轉運途徑。相關論文於2020年4月8日在線發表在《細胞》雜誌上。
研究人員發現了一條調節囊泡進入和無導向貨物分泌的轉運途徑。研究人員將TMED10鑑定為許多無導向貨物的囊泡進入和分泌的蛋白質通道。TMED10 C末端區域與貨物中基序的相互作用說明了貨物的選擇性釋放。
在體外重建測定中，TMED10直接介導無導向貨物進入脂質體的膜轉運，這取決於蛋白的解摺疊和HSP90的增強。在細胞中，TMED10位於內質網與高爾基體的中間隔室，並引導貨物進入該隔室。此外，貨物引起了TMD10同源寡聚體的形成，其可以充當貨物轉運的蛋白質通道。
據了解，許多缺乏信號肽的胞質蛋白（稱為無導向貨物）是通過非常規分泌方式分泌的。囊泡運輸是其中主要途徑。目前尚不清楚無導向貨物如何進入囊泡。
附：英文原文
Title: A Translocation Pathway for Vesicle-Mediated Unconventional Protein Secretion
Author: Min Zhang, Lei Liu, Xubo Lin, Yang Wang, Ying Li, Qing Guo, Shulin Li, Yuxin Sun, Xuan Tao, Di Zhang, Xiachen Lv, Li Zheng, Liang Ge
Issue&Volume: 2020-04-08
Abstract: Many cytosolic proteins lacking a signal peptide, called leaderless cargoes, are secretedthrough unconventional secretion. Vesicle trafficking is a major pathway involved.It is unclear how leaderless cargoes enter into the vesicle. Here, we find a translocationpathway regulating vesicle entry and secretion of leaderless cargoes. We identifyTMED10 as a protein channel for the vesicle entry and secretion of many leaderlesscargoes. The interaction of TMED10 C-terminal region with a motif in the cargo accountsfor the selective release of the cargoes. In an in vitro reconstitution assay, TMED10 directly mediates the membrane translocation of leaderlesscargoes into the liposome, which is dependent on protein unfolding and enhanced byHSP90s. In the cell, TMED10 localizes on the endoplasmic reticulum (ER)-Golgi intermediatecompartment and directs the entry of cargoes into this compartment. Furthermore, cargoinduces the formation of TMED10 homo-oligomers which may act as a protein channelfor cargo translocation.
DOI: 10.1016/j.cell.2020.03.031
Source: https://www.cell.com/cell/fulltext/S0092-8674(20)30323-8
Cell：《細胞》，創刊於1974年。隸屬於細胞出版社，最新IF：36.216