科學家繪製出單細胞解析度下人類腸道發育的時空圖譜
作者:
小柯機器人發布時間:2021/1/6 17:36:06
英國牛津大學Alison Simmons、HashemKoohy等研究人員合作繪製出單細胞解析度下人類腸道發育的時空圖譜。該研究於2021年1月5日在線發表於國際一流學術期刊《細胞》。
研究人員整合了單細胞RNA測序和空間轉錄組學來表徵隨時間變化的腸道形態發生。研究人員確定了101個細胞狀態,包括上皮和間充質祖細胞群以及與關鍵形態發生學相關的程序。研究人員描述了隱窩-絨毛軸的形成,神經、血管、間充質的形態發生以及發育中的腸道免疫群體。
研究人員確定了發育為成纖維細胞和成肌纖維細胞亞型的分化層次,並描述了這些功能,包括血管微環境細胞的多種功能。研究人員揭示了Peyer斑和腸道相關淋巴組織(GALT)的起源,並描述了特定於位置的免疫程序。研究人員使用這一資源來呈現了形態發生素梯度的無偏倚分析,這一梯度能夠指導細胞分化的連續波並定義與罕見發育性腸道疾病相關的細胞和位置。
研究人員彙編了一個公開可用的在線資源,即胎兒腸道發育的時空分析資源(STAR-FINDer),以希望促進今後的研究工作。
附:英文原文
Title: Spatiotemporal analysis of human intestinal development at single-cell resolution
Author: David Fawkner-Corbett, Agne Antanaviciute, Kaushal Parikh, Marta Jagielowicz, Ana Sousa Gerós, Tarun Gupta, Neil Ashley, Doran Khamis, Darren Fowler, Edward Morrissey, Chris Cunningham, Paul R.V. Johnson, Hashem Koohy, Alison Simmons
Issue&Volume: 2021-01-05
Abstract: Development of the human intestine is not well understood. Here, we link single-cell RNA sequencing and spatial transcriptomics to characterize intestinal morphogenesis through time. We identify 101 cell states including epithelial and mesenchymal progenitor populations and programs linked to key morphogenetic milestones. We describe principles of crypt-villus axis formation; neural, vascular, mesenchymal morphogenesis, and immune population of the developing gut. We identify the differentiation hierarchies of developing fibroblast and myofibroblast subtypes and describe diverse functions for these including as vascular niche cells. We pinpoint the origins of Peyer’s patches and gut-associated lymphoid tissue (GALT) and describe location-specific immune programs. We use our resource to present an unbiased analysis of morphogen gradients that direct sequential waves of cellular differentiation and define cells and locations linked to rare developmental intestinal disorders. We compile a publicly available online resource, spatio-temporal analysis resource of fetal intestinal development (STAR-FINDer), to facilitate further work.
DOI: 10.1016/j.cell.2020.12.016
Source: https://www.cell.com/cell/fulltext/S0092-8674(20)31686-X
Cell:《細胞》,創刊於1974年。隸屬於細胞出版社,最新IF:36.216