單細胞測序破譯人類睪丸的發育過程
作者：
小柯機器人發布時間：2021/1/18 12:30:14
美國猶他大學Bradley R. Cairns等研究人員合作利用單細胞測序解析人類睪丸的發育過程。相關論文於2021年1月15日在線發表在《細胞—幹細胞》雜誌上。
研究人員表示，人類睪丸的發育涉及種系和體細胞身份的複雜變化。
為了更好地理解，研究人員對來自胚胎、胎兒和嬰兒階段睪丸細胞的約32,500個單細胞轉錄組進行了分析。數據顯示，在受精後6-7周，隨著睪丸線的建立，Sertoli和間質細胞起源於一個共同的異質祖細胞，然後匯集成胎兒Sertoli細胞（表達成管基因）或間質細胞（包括表達類固醇生成基因的Leydig譜系細胞）。從受精後14–16周開始，男性原始生殖細胞退出有絲分裂，下調多能轉錄因子，並轉變成與最初在嬰兒和成人睪丸中定義的精原細胞狀態0非常相似的細胞。因此，研究人員稱這些胎兒精原細胞為「狀態f0」。
總體而言，這項工作揭示了對胚胎、胎兒和產後雄性生殖系以及軀體微環境發育的多種見解。
附：英文原文
Title: Single-cell analysis of the developing human testis reveals somatic niche cell specification and fetal germline stem cell establishment
Author: Jingtao Guo, Enrique Sosa, Tsotne Chitiashvili, Xichen Nie, Ernesto Javier Rojas, Elizabeth Oliver, Kathrin Plath, James M. Hotaling, Jan-Bernd Stukenborg, Amander T. Clark, Bradley R. Cairns
Issue&Volume: 2021-01-15
Abstract: Human testis development in prenatal life involves complex changes in germline andsomatic cell identity. To better understand, we profiled and analyzed ～32,500 single-celltranscriptomes of testicular cells from embryonic, fetal, and infant stages. Our datashow that at 6–7 weeks postfertilization, as the testicular cords are established,the Sertoli and interstitial cells originate from a common heterogeneous progenitorpool, which then resolves into fetal Sertoli cells (expressing tube-forming genes)or interstitial cells (including Leydig-lineage cells expressing steroidogenesis genes).Almost 10 weeks later, beginning at 14–16 weeks postfertilization, the male primordialgerm cells exit mitosis, downregulate pluripotent transcription factors, and transitioninto cells that strongly resemble the state 0 spermatogonia originally defined inthe infant and adult testes. Therefore, we called these fetal spermatogonia 「statef0.」 Overall, we reveal multiple insights into the coordinated and temporal developmentof the embryonic, fetal, and postnatal male germline together with the somatic niche.
DOI: 10.1016/j.stem.2020.12.004
Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(20)30588-9