皮質GABA能細胞的整合形態和轉錄組分型
作者：
小柯機器人發布時間：2020/11/14 23:37:45
皮質GABA能細胞的整合形態和轉錄組分型，這一成果由美國艾倫腦科學研究所Gabe J. Murphy、Nathan W. Gouwens和Staci A. Sorensen研究小組經過不懈努力而取得。 這一研究成果發表在2020年11月12日出版的國際學術期刊《細胞》上。
為了更好地對神經元細胞類型進行分類，研究人員表徵了4,200多個小鼠視覺皮層GABA能中間神經元的轉錄組和內在生理特性，並重建了這些神經元中517個局部形態。研究發現大多數轉錄組類型（t型）佔據視覺皮層內的特定層流位置，並且對於大多數類型，映射到t型的細胞表現出一致的電生理和形態學特性。這些屬性表明t型神經元之間的離散和連續變化。
通過多模式綜合分析，研究人員確定了28種met-type，它們具有一致的形態學、電生理學和轉錄組學特性，並且具有較強的相互可預測性。研究人員將特定皮層軸突神經支配識別模式作為區分不同met細胞類型的標誌。這些met型代表了皮質GABA能中間神經元類型的統一標準。該研究提供了一個系統性網絡以實現對現有知識和將來跨不同模式分析的橋接。
據了解，通常根據結構、生理或遺傳屬性的差異將神經元分為不同類型。
附：英文原文
Title: Integrated Morphoelectric and Transcriptomic Classification of Cortical GABAergic Cells
Author: Nathan W. Gouwens, Staci A. Sorensen, Fahimeh Baftizadeh, Agata Budzillo, Brian R. Lee, Tim Jarsky, Lauren Alfiler, Katherine Baker, Eliza Barkan, Kyla Berry, Darren Bertagnolli, Kris Bickley, Jasmine Bomben, Thomas Braun, Krissy Brouner, Tamara Casper, Kirsten Crichton, Tanya L. Daigle, Rachel Dalley, Rebecca A. de Frates, Nick Dee, Tsega Desta, Samuel Dingman Lee, Nadezhda Dotson, Tom Egdorf, Lauren Ellingwood, Rachel Enstrom, Luke Esposito, Colin Farrell, David Feng, Olivia Fong, Rohan Gala, Clare Gamlin, Amanda Gary, Alexandra Glandon, Jeff Goldy, Melissa Gorham, Lucas Graybuck, Hong Gu, Kristen Hadley, Michael J. Hawrylycz, Alex M. Henry, DiJon Hill, Madie Hupp, Sara Kebede, Tae Kyung Kim, Lisa Kim, Matthew Kroll, Changkyu Lee, Katherine E. Link, Matthew Mallory, Rusty Mann, Michelle Maxwell, Medea McGraw, Delissa McMillen, Alice Mukora, Lindsay Ng, Lydia Ng, Kiet Ngo, Philip R. Nicovich, Aaron Oldre, Daniel Park, Hanchuan Peng, Osnat Penn, Thanh Pham, Alice Pom, Zoran Popovi, Lydia Potekhina, Ramkumar Rajanbabu
Issue&Volume: 2020/11/12
Abstract: Neurons are frequently classified into distinct types on the basis of structural, physiological, or genetic attributes. To better constrain the definition of neuronal cell types, we characterized the transcriptomes and intrinsic physiological properties of over 4,200 mouse visual cortical GABAergic interneurons and reconstructed the local morphologies of 517 of those neurons. We find that most transcriptomic types (t-types) occupy specific laminar positions within visual cortex, and, for most types, the cells mapping to a t-type exhibit consistent electrophysiological and morphological properties. These properties display both discrete and continuous variation among t-types. Through multimodal integrated analysis, we define 28 met-types that have congruent morphological, electrophysiological, and transcriptomic properties and robust mutual predictability. We identify layer-specific axon innervation pattern as a defining feature distinguishing different met-types. These met-types represent a unified definition of cortical GABAergic interneuron types, providing a systematic framework to capture existing knowledge and bridge future analyses across different modalities.
DOI: 10.1016/j.cell.2020.09.057
Source: https://www.cell.com/cell/fulltext/S0092-8674(20)31254-X
Cell：《細胞》，創刊於1974年。隸屬於細胞出版社，最新IF：36.216