Effect of Bilberry Extract on Development of Form-Deprivation Myopia in the Guinea Pig
J Ocul Pharmacol Ther. 2016 Mar 16. [Epub ahead of print]
Hong-Wei Deng,1,* Yuan Tian,2,* Xiao-Juan Zhou, 2 Xiao-Ming Zhang, 2 and Jing Meng2
Abstract
Purpose: To investigate the effects of bilberry extract, Difrarel, on form-deprivation myopia in guinea pigs. Method: Thirty healthy pigmented guinea pigs, at the age of 3 weeks, were randomly assigned to 3 groups: 2 groups receiving daily orally administered Difrarel (300mg/kg) either from the outset for 4 or 2 weeks after form deprivation and a control group. Form deprivation was induced with translucent eye patches on the right eye and lasted for 4 weeks. Another 12 guinea pigs without the form deprivation were assigned into 2 groups for oral administration of Difrarel and saline for 4 weeks. The refractive errors and axial length were measured in both eyes, and the posterior sclera was removed and assayed to evaluate the expressions of matrix metalloproteinase 2 (MMP2) and collagen I by a quantitative polymerase chain reaction and Western blot.
Results: Oral administration of Difrarel for 4 weeks was found to inhibit the axial elongation and decrease the myopic shift of refractive errors in the form-deprived eye. Upregulation of MMP2 and degradation of collagen I in the sclera of form-deprived eye were observed, and these were prevented by orally administered Difrarel. Moreover, oral administration of Difrarel had no effects on axial length, refractive errors, and the expression of MMP2 in the normal eye.
Conclusion: This work demonstrates that orally administered Difrarel has an effect against form-deprivation myopia and raises the possibility that bilberry extracts or specific components might represent a useful treatment for preventing the progression of myopia in clinical practice。
《越橘果提取物對豚鼠形覺剝奪性近視進展的作用機制》
譯文：本文主要探討遞法明中的越橘果提取物對豚鼠形覺剝奪性近視的作用。
方法：選取健康3周齡豚鼠 30 只隨機分為2組，其中2組分別於建立模型開始時、2周後，接受每日口服遞法明中的越橘果提取物 (300 mg/kg)；另外一組作為對照。所有豚鼠均用半透明眼罩遮蓋右眼，遮蓋時間持續4周。另外12隻不建立形覺剝奪模型的豚鼠，被隨機分為2組，分別口服遞法明中的越橘果提取物和生理鹽水，持續4周。測量雙眼屈光度及眼軸長度，取後極部鞏膜行實時定量基因擴增螢光法、蛋白印跡法檢測MMP-2 與Collagen I 的表達。
結果：口服遞法明中的越橘果提取物4周可抑制形覺剝奪眼的眼軸增長、近視屈光度改變。剝奪眼鞏膜組織中觀察到MMP-2表達上調、collagen I降解，口服遞法明中的越橘果提取物可阻止這些改變。此外，遞法明中的越橘果提取物對正常眼眼軸長度、屈光度和MMP-2的表達沒有影響。結論：遞法明中的越橘果提取物花青素能抑制豚鼠形覺剝奪性近視的形成，遞法明中的越橘果提取物或其特殊成分可能為臨床上阻止近視進展提供有效方法。