研究揭示vHPC網絡迴路和分子結構
作者：
小柯機器人發布時間：2020/9/15 22:05:33
美國加州大學Mazen A. Kheirbek研究組取得最新進展。他們揭示了腹側海馬（vHPC）網絡的迴路和分子結構。相關論文發表在2020年9月14日出版的《自然-神經科學》雜誌上。
他們使用病毒和測序方法來定義擴展腹側CA1（vCA1）迴路的邏輯。使用遺傳條形碼神經元（MAPseq）的高通量測序來繪製成千上萬個vCA1神經元的軸突投影，他們確定了一群神經元，它們同時向多個已知區域擴散信息，以調節壓力軸和避免接觸行為。通過vCA1投射神經元的分子譜分析和病毒輸入-輸出示蹤，他們展示了具有不同投射靶標的神經元在輸入和轉錄特徵上如何表現不同。
這些研究揭示了vCA1新的組織原則，可能是其功能異質性的基礎。
研究人員表示，vHPC是處理情感信息的網絡中的重要樞紐。儘管最近的研究表明vCA1投射神經元在功能上是可分離的，但如何組織vCA1的輸入和輸出的基本原理仍不清楚。
附：英文原文
Title: Circuit and molecular architecture of a ventral hippocampal network
Author: Mark M. Gergues, Kasey J. Han, Hye Sun Choi, Brandon Brown, Kelsey J. Clausing, Victoria S. Turner, Ilia D. Vainchtein, Anna V. Molofsky, Mazen A. Kheirbek
Issue&Volume: 2020-09-14
Abstract: The ventral hippocampus (vHPC) is a critical hub in networks that process emotional information. While recent studies have indicated that ventral CA1 (vCA1) projection neurons are functionally dissociable, the basic principles of how the inputs and outputs of vCA1 are organized remain unclear. Here, we used viral and sequencing approaches to define the logic of the extended vCA1 circuit. Using high-throughput sequencing of genetically barcoded neurons (MAPseq) to map the axonal projections of thousands of vCA1 neurons, we identify a population of neurons that simultaneously broadcast information to multiple areas known to regulate the stress axis and approach–avoidance behavior. Through molecular profiling and viral input–output tracing of vCA1 projection neurons, we show how neurons with distinct projection targets may differ in their inputs and transcriptional signatures. These studies reveal new organizational principles of vCA1 that may underlie its functional heterogeneity.
DOI: 10.1038/s41593-020-0705-8
Source: https://www.nature.com/articles/s41593-020-0705-8