研究揭示全基因組範圍的重複基因保留
作者:
小柯機器人發布時間:2020/6/28 14:39:01
加拿大多倫多大學Charles Boone、Brenda J. Andrews、美國明尼蘇達大學Chad L. Myers等研究人員合作利用複雜的遺傳相互作用分析,揭示了全基因組範圍重複基因的保留。2020年6月26日,《科學》發表了這一成果。
研究人員報導了一個包含來自全基因組複製事件的酵母旁系同源物的系統型複雜遺傳相互作用分析。每個旁系同源物缺失突變體的雙基因相互作用映射,以及雙突變體的三基因相互作用映射結果對其作用以及對其功能冗餘的定量檢測提供了新的見解。三基因相互作用分析可區分兩類旁系同源物:功能上分歧較大的類群以及另一種保留更多功能重疊的類群。基因特徵分析和建模表明,重複基因的進化軌跡是由功能和結構的糾纏因素共同決定的。
據了解,全基因組複製在包括人類基因組在內的許多生物的基因組進化中發揮了核心作用。大多數重複的基因被消除,影響長期重複保留的因素仍然知之甚少。
附:英文原文
Title: Exploring whole-genome duplicate gene retention with complex genetic interaction analysis
Author: Elena Kuzmin, Benjamin VanderSluis, Alex N. Nguyen Ba, Wen Wang, Elizabeth N. Koch, Matej Usaj, Anton Khmelinskii, Mojca Mattiazzi Usaj, Jolanda van Leeuwen, Oren Kraus, Amy Tresenrider, Michael Pryszlak, Ming-Che Hu, Brenda Varriano, Michael Costanzo, Michael Knop, Alan Moses, Chad L. Myers, Brenda J. Andrews, Charles Boone
Issue&Volume: 2020/06/26
Abstract: Whole-genome duplication has played a central role in the genome evolution of many organisms, including the human genome. Most duplicated genes are eliminated, and factors that influence the retention of persisting duplicates remain poorly understood. We describe a systematic complex genetic interaction analysis with yeast paralogs derived from the whole-genome duplication event. Mapping of digenic interactions for a deletion mutant of each paralog, and of trigenic interactions for the double mutant, provides insight into their roles and a quantitative measure of their functional redundancy. Trigenic interaction analysis distinguishes two classes of paralogs: a more functionally divergent subset and another that retained more functional overlap. Gene feature analysis and modeling suggest that evolutionary trajectories of duplicated genes are dictated by combined functional and structural entanglement factors.
DOI: 10.1126/science.aaz5667
Source: https://science.sciencemag.org/content/368/6498/eaaz5667
Science:《科學》,創刊於1880年。隸屬於美國科學促進會,最新IF:41.037