NMT歷史上的今天
018年8月6日，北京林業大學尹偉倫、孟森在International journal of molecular sciences上發表了標題為Distinct Carbon and Nitrogen Metabolism of Two Contrasting Poplar Species in Response to Different N Supply Levels的文章。
2012年8月6日，中科院海洋所王廣策、林阿朋在Physiologia Plantarum上發表了標題為Simultaneous measurements of H+and O2 fluxes in Zostera marina and its physiological implications 的文章。
期刊：International journal of molecular sciences
主題：兩種楊樹對不同氮素供應水平的碳氮代謝差異
標題：Distinct Carbon and Nitrogen Metabolism of Two Contrasting Poplar Species in Response to Different N Supply Levels
影響因子：3.687
檢測指標：NH4+、NO3-、H+流速流速
檢測部位：楊樹根部
NH4+、NO3-、H+流實驗處理方法：
楊樹在低中高（0.01,1或10 mM的NH4NO3）的氮濃度下處理4周
NH4+、NO3-、H+流實驗測試液成份：
0.01mM/1mM/10mM NH4NO3，0.1mM KCl，0.1mM CaCl 2，pH 5.5
通訊作者：北京林業大學尹偉倫、孟森
英文摘要
Poplars have evolved various strategies to optimize acclimation responses to environmental conditions. However, how poplars balance growth and nitrogen deficiency remains to be elucidated.
In the present study, changes in root development, carbon and nitrogen physiology, and the transcript abundance of associated genes were investigated in slow-growing Populus simonii (Ps) and fast-growing Populus euramericana (Pe) saplings treated with low, medium, and high nitrogen supply.
The slow-growing Ps showed a flourishing system, higher δ15N, accelerated C export, lower N uptake and assimilation, and less sensitive transcriptional regulation in response to low N supply.
The slow-growing Ps also had greater resistance to N deficiency due to the transport of photosynthate to the roots and the stimulation of root development, which allows survival.
To support its rapid metabolism and growth, compared with the slow-growing Ps, the fast-growing Pe showed greater root development, C/N uptake and assimilation capacity, and more responsive transcriptional regulation with greater N supply.
These data suggest that poplars can differentially manage C/N metabolism and photosynthate allocation under different N supply conditions.
中文摘要（谷歌機翻）
楊樹已經發展出各種策略來優化對環境條件的適應性反應。然而，楊樹如何平衡生長和氮缺乏仍有待闡明。在本研究中，在慢生長的小葉楊（Pops simonii）（Ps）和快速生長的歐洲楊（Populus euramericana（Pe）幼樹）中研究了根系發育，碳和氮生理學以及相關基因的轉錄本豐度的變化。高氮供應。緩慢生長的Ps顯示出繁殖系統，更高的δ15N，加速的C輸出，更低的N吸收和同化，以及響應於低N供應的較不敏感的轉錄調節。由於光合產物向根部的轉運和根系發育的刺激，緩慢生長的Ps對N缺乏具有更大的抗性，這允許存活。為了支持其快速代謝和生長，與生長緩慢的Ps相比，快速生長的Pe顯示出更大的根發育，C / N吸收和同化能力，以及具有更大N供應的更具響應性的轉錄調節。這些數據表明，楊樹可以在不同的氮供應條件下差異化地控制C / N代謝和光合產物分配。
Figure 4. Net NH4+(A), NO3(B) and H+(C) fluxes of P. simonii (Ps) and Populus euramericana (Pe) under 0.01, 1 and 10 mM NH4NO3. Bars labelled with different letters indicate significant difference between the treatments. p-Values of the ANOVAs of species, N treatment, and their interaction are indicated. ** p < 0.01; *** p < 0.001; ns, not significant.
期刊：Physiologia Plantarum
主題：同時測量H+和O2流速及其生理意義
標題：Simultaneous measurements of H+ and O2 fluxes in Zostera marina and its physiological implications
影響因子：3.067
檢測指標：H+、O2流速
檢測部位：大葉藻葉片
H+、O2流實驗處理方法：
大葉藻葉片瞬時由暗至光（300μmolm-2s-1）處理
H+、O2流實驗測試液成份：
天然海水（NSW）鹽濃度30–32‰
通訊作者：中科院海洋所王廣策、林阿朋
英文摘要
Zostera marina (eelgrass) is an important ecological component of many shallow, temperate lagoons. Evidence suggests that Z. marina has a high bicarbonate utilization capability, which could be promoted by possible proton extrusion and the consequent formation of an 『acid zone』 in the apoplastic space (unstirred layer) of its leaves.
It has been found that 50mM of the buffer Tris significantly inhibited the photosynthetic O2 evolution of Z. marina and it was proposed that this was because of Tris's ability to bond with protons outside the cell wall.
To investigate if H+ played an important role in the photosynthetic carbon utilization of Z. marina, it is very important to simultaneously monitor the photosynthesis status and possible H+ fluxes. However, probably because of the lack of suitable techniques, this has never been attempted.
In this study, experiments were undertaken on Z. marina by monitoring H+ and O2 fluxes and the relative electron transport rates during light–dark transition. During stable photosynthesis, in addition to an obvious O2 outflow, there was a significant net H+ influx connected to Z.
marina photosynthesis. The inhibitory effects of both Tris and respiration inhibitors on apparent O2 evolution of Z. marina were confirmed. However, evidence did not support the proposed Tris inhibition mechanism.
中文摘要（谷歌機翻）
Zostera marina（eelgrass）是許多淺水溫帶瀉湖的重要生態組成部分。有證據表明，Z。marina具有較高的碳酸氫鹽利用能力，可以通過可能的質子擠出促進，並因此在其葉片的非質外體空間（未攪拌層）中形成「酸性區域」。已經發現50mM的緩衝液Tris顯著抑制了Z.marina的光合作用O2進化，並且提出這是因為Tris能夠與細胞壁外的質子結合。為了研究H +是否在Z. marina的光合碳利用中起重要作用，同時監測光合作用狀態和可能的H +通量是非常重要的。然而，可能由於缺乏合適的技術，這從未嘗試過。在這項研究中，通過監測H +和O2通量以及在明暗過渡期間的相對電子傳輸速率對Z.marina進行了實驗。在穩定的光合作用期間，除了明顯的O2流出外，還有顯著的淨H +流入與Z. marina光合作用相關。證實了Tris和呼吸抑制劑對Z. marina的表觀O2進化的抑制作用。然而，證據不支持所提出的Tris抑制機制。
Fig. 1. Typical transient changes in H+ux near Zostera marina leaf segments in response to bright white light. The result of a single measurement is used in this gure as multiple results in one graph would result in large uctuations and mask the transient responses.
The sample was dark adapted for >1 h. After 25 min of measurement, leaf segments were exposed to bright white light (300 μmol m2 s1)for 15 min. Measurements were made at intervals of 6.4 s. Each point on the graph represents average data over an interval of three measurements.