抗體親和力決定記憶和生發中心B細胞命運的選擇
作者：
小柯機器人發布時間：2020/10/31 20:39:25
美國洛克菲勒大學Michel C. Nussenzweig小組在研究中取得進展。他們的研究發現抗體親和力決定記憶和生發中心(GC)B細胞命運的選擇。相關論文在線發表在2020年10月29日出版的《細胞》雜誌上。
研究人員利用譜系示蹤和單個B細胞克隆抗體相結合的方法，檢測了親和力在B細胞選擇進入生發中心或進入HIV-1抗原免疫小鼠的記憶B細胞區室中的作用。研究發現，同時生成的記憶和GC B細胞在整個免疫反應中對抗原的親和力不同。GC及其前體細胞具有強抗原結合能力，而記憶B細胞則沒有。
因此，多克隆記憶B細胞區室是由在免疫反應期間被激活但其抗原結合親和力無法支持GC中進一步克隆擴展的B細胞組成。
據悉，免疫記憶是防止反覆感染所必需的，並且是所有有效疫苗起作用的基礎。記憶B細胞產生的抗體在許多此類反應中起著至關重要的作用。
附：英文原文
Title: Antibody Affinity Shapes the Choice between Memory and Germinal Center B Cell Fates
Author: Charlotte Viant, Georg H.J. Weymar, Amelia Escolano, Spencer Chen, Harald Hartweger, Melissa Cipolla, Anna Gazumyan, Michel C. Nussenzweig
Issue&Volume: 2020-10-29
Abstract: Immunological memory is required for protection against repeated infections and isthe basis of all effective vaccines. Antibodies produced by memory B cells play anessential role in many of these responses. We have combined lineage tracing with antibodycloning from single B cells to examine the role of affinity in B cell selection intogerminal centers (GCs) and the memory B cell compartment in mice immunized with anHIV-1 antigen. We find that contemporaneously developing memory and GC B cells differin their affinity for antigen throughout the immune response. Whereas GC cells andtheir precursors are enriched in antigen binding, memory B cells are not. Thus, thepolyclonal memory B cell compartment is composed of B cells that were activated duringthe immune response but whose antigen binding affinity failed to support further clonal expansion in the GC.
DOI: 10.1016/j.cell.2020.09.063
Source: https://www.cell.com/cell/fulltext/S0092-8674(20)31304-0
Cell：《細胞》，創刊於1974年。隸屬於細胞出版社，最新IF：36.216