噬菌體聯合方案可用於防治番茄青枯病
作者:
小柯機器人發布時間:2019/12/3 12:55:01
南京農業大學沈其榮、韋中、Ville-Petri Friman等研究人員合作提出了針對番茄青枯病的噬菌體聯合治療方案。這一研究成果於12月2日在線發表於《自然—生物技術》。
研究人員研究了不同噬菌體組合對番茄青枯雷爾氏菌感染的生物防治功效。在單個作物季節的溫室和田間試驗中,增加組合噬菌體的數量可使疾病發生率降低多達80%。
疾病發生率降低的原因是病原體密度降低,以及篩選出了噬菌體抗藥性但生長緩慢的病原體菌株,並且對青枯菌拮抗的細菌種類的富集。噬菌體處理不影響現有的根際微生物群。特定的噬菌體組合具有作為控制植物病原細菌工具的潛力。
據介紹,噬菌體被認為是可選的殺蟲劑用於清除農作物的細菌病原體。但是,噬菌體生物防治的功效是不確定的,並且在自然的根際微生物群落中知之甚少。
附:英文原文
Title: Phage combination therapies for bacterial wilt disease in tomato
Author: Xiaofang Wang, Zhong Wei, Keming Yang, Jianing Wang, Alexandre Jousset, Yangchun Xu, Qirong Shen, Ville-Petri Friman
Issue&Volume: 2019-12-02
Abstract: Bacteriophages have been proposed as an alternative to pesticides to kill bacterial pathogens of crops. However, the efficacy of phage biocontrol is variable and poorly understood in natural rhizosphere microbiomes. We studied biocontrol efficacy of different phage combinations on Ralstonia solanacearum infection in tomato. Increasing the number of phages in combinations decreased the incidence of disease by up to 80% in greenhouse and field experiments during a single crop season. The decreased incidence of disease was explained by a reduction in pathogen density and the selection for phage-resistant but slow-growing pathogen strains, together with enrichment for bacterial species that were antagonistic toward R. solanacearum. Phage treatment did not affect the existing rhizosphere microbiota. Specific phage combinations have potential as precision tools to control plant pathogenic bacteria.
DOI: 10.1038/s41587-019-0328-3
Source: https://www.nature.com/articles/s41587-019-0328-3