研學 | 生物化工類多糖方向頂刊推薦

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Structural characterization, antioxidant and antibacterial activities of a novel polysaccharide from Periploca laevigata root barks 

槓柳根皮多糖的結構表徵、抗氧化和抗菌活性

化學 2區、高分子科學 2區、應用化學 1區、有機化學 2區  IF:5.518

Hajji, M., Hamdi, M., Sellimi, S., Ksouda, G., Laouer, H., Li, S., & Nasri, M. Structural characterization, antioxidant and antibacterial activities of a novel polysaccharide from Periploca laevigata root barks. Carbohydrate Polymers[J].2018. doi:10.1016/j.carbpol.2018.11.020

論文連結：https://www.sciencedirect.com/science/article/pii/S0144861718313523

摘要：

🧠A novel water-soluble polysaccharide named PLP1 was successfully isolated from root barks of Periploca laevigata by hot water extraction and further purified by DEAE-sepharose chromatography. PLP1 has a relative molar mass of 5.57 105 g/mol determined by Ultra-high performance liquid chromatography (UHPLC). The PLP1 structure was investigated by chemical and instrumental analysis including gas chromatography mass spectrometry (GC-MS), methylation analysis, Fourier transform infrared (FT-IR) spectroscopy and nuclear magnetic spectroscopy (1H-NMR and 13C-NMR). Results showed that PLP1 was mainly composed of glucose, galactose, mannose and glucuronic acid in a molar percent of 62.9, 19.4, 11.8 and 5.9, respectively. The backbone of PLP1 was composed of 1,3-β-D-Glcp, 1,3,4-α-D-Glcp, 1,4-α-D-Manp, 1,6-α-D-Manp, 1,3-α-D-Galp and 1,6-β-D-Galp. The thermogravimetry analysis (TGA) and the differential scanning calorimetry (DSC) were used and showed that PLP1 has good thermal stability under 229 °C. Moreover, the purified polysaccharide demonstrated an appreciable in vitro antioxidant potential and high antibacterial activity against several Gram (+) and Gram (-) strains. These finding suggested that PLP1 might be suitable for use as functional foods and as potential therapeutic agents.

🧠採用熱水提取法從槓柳根皮中分離到一種新的水溶性多糖PLP1，並用DEAE-瓊脂糖層析法進一步純化。超高效液相色譜法（UHPLC）測定PLP1的相對摩爾質量為5.57 105 g/mol。採用氣相色譜-質譜(GC-MS)、甲基化分析、傅立葉變換紅外光譜(FT-IR)和核磁共振(1H-NMR和13C-NMR)等分析手段對PLP1的結構進行了研究。結果表明，PLP1主要由葡萄糖、半乳糖、甘露糖和葡萄糖醛酸組成，其摩爾分數分別為62.9、19.4、11.8和5.9；PLP1的骨架由1,3-β-D-Glcp、1,3,4-α-D-Glcp、1,4-α-D-Manp、1,6-α-D-Manp、1,3-α-D-Galp和1,6-β-D-Galp組成。採用熱重分析(TGA)和差示掃描量熱法(DSC)對PLP1進行了鑑定,結果表明PLP1在229℃下具有良好的熱穩定性,純化多糖的體外抗氧化能力強,對Evalar Gram（+）和Gram（-）菌株的抗菌活性高。這些發現表明PLP1可能適合用作功能性食品和潛在的治療劑。

Recent developments in Hericium erinaceus polysaccharides: extraction, purification, structural characteristics and biological activities 

猴頭菇多糖的最新進展：提取，純化，結構特徵和生物活性 

食品科學1區  IF:6.015

Xiao-Yin Wang, Duo-duo Zhang, Jun-Yi Yin, Shao-Ping Nie, Ming-Yong Xie.Recent developments in Hericium erinaceus polysaccharides: extraction, purification, structural characteristics and biological activities. Critical Reviews in Food Science and Nutrition [J].2018. doi:10.1080/10408398.2018.1521370

論文連結：

http://www.x-mol.com/paper/889269

摘要：

🧠Hericium erinaceus (H. erinaceus), an edible mushroom with medicinal value, has a long history of usage in China and other oriental countries. Polysaccharide is supposed to be one of the major bioactive compounds in H. erinaceus, which possesses immunomodulating, anti-cancer, antioxidant, gastroprotection and intestinal health promotion, neuroprotective, hepatoprotective, antihpyerglycemic and hypolipidemic activities. In this review, the current advancements on extraction, purification, structural characteristics and biological activities of polysaccharide from different sources (fruiting body, mycelium and culture broth) of H. erinaceus were summarized. Among these aspects, summaries of the structural characteristics focused on the purified polysaccharides. Meanwhile, comparisons on the structural characteristics among the purified polysaccharides obtained from above three sources were made. Moreover, their biological activities were introduced on the basis of in vivo and in vitroexperiments, and some possible action mechanisms were listed. Furthermore, the structure-activity relationship of the polysaccharide was discussed. New perspectives for the future work of Hericium erinaceus polysaccharide were also proposed.

🧠猴頭菇（Hericium erinaceus）是一種具有藥用價值的食用菌，在中國和其他東方國家有著悠久的使用歷史。多糖被認為是H.ininaceus中主要的生物活性化合物之一，具有免疫調節，抗癌，抗氧化，胃保護和腸道健康促進，神經保護，保肝，抗高血糖和降血脂活性。在這篇綜述中，對不同來源的多糖（子實體，菌絲體和培養液）在提取，純化，結構特徵和生物活性方面進行當前進展總結。其中，集中於對純化多糖進行結構鑑定。同時，對從上述三種來源獲得的純化多糖的結構特徵進行了比較。此外，在體內和體外實驗的基礎上引入活性評價研究，並列出了一些可能的作用機制。此外，還討論了多糖的結構 - 活性關係。還提出了猴頭菇多糖未來工作的新觀點。

Injectable and self-healing polysaccharide-based hydrogel for pH-responsive drug release

可注射和自愈的多糖水凝膠用於pH響應性藥物釋放

生物化學1區  IF:3.909

Qian, C., Zhang, T., Gravesande, J., Baysah, C., Song, X., & Xing, J. Injectable and self-healing polysaccharide-based hydrogel for pH-responsive drug release. International Journal of Biological Macromolecules[J].2018. doi:10.1016/j.ijbiomac.2018.11.048

論文連結：

sci-hub.tw/10.1016/j.ijbiomac.2018.11.048

摘要：

🧠Injectable hydrogels with self-healing and pH-responsive property are appealing for biomedical applications. Herein, we developed a facile and green method to prepare a multifunctional polysaccharide-based hydrogel as a new carrier of drug. The hydrogels were prepared by forming reversible chemical bond between carboxyethyl-modified chitosan (CEC) and aldehyde modified hyaluronic acid (A-HA). The morphology and rheological property of the hydrogels with different solid content were systematically characterized. Owing to the dynamic equilibrium of the Schiff base bonds between amine groups on CEC and aldehyde groups on A-HA, the rapid self-healing performance of hydrogels was confirmed through qualitative and quantitative methods without any external stimulus. The pH-responsive behaviour was demonstrated by equilibrium swelling and in vitro Doxorubicin (Dox) release in PBS medium with various pH. In acidic condition, Dox can be release more rapidly compared with weak alkaline medium. Furthermore, the kill effect of Dox released from hydrogels for cancer cells was investigated. In vitro degradation and cytotoxicity examinations showed that the hydrogel is biodegradable and biocompatible. Therefore, such polysaccharide-based injectable self-healing and pH-responsive hydrogel is a promising candidate as drug delivery carrier.

🧠具有自愈性和pH響應性的可注射水凝膠對生物醫學應用具有吸引力。在此，我們發展了一種簡便且綠色的方法，以製備多功能多糖基水凝膠作為新的藥物載體。採用羧乙基改性殼聚糖（CEC）與醛改性透明質酸（A-HA）形成可逆化學鍵的方法製備了水凝膠。對不同固含量的水凝膠的形態和流變性能進行了系統的表徵。由於CEC上的胺基與A-HA上的醛基之間的Schiff鹼鍵的動態平衡，在沒有任何外界刺激的情況下，通過定性和定量方法證實了水凝膠的快速自癒合性能。在不同pH的PBS介質中，通過平衡溶脹和體外釋放多柔比星（Dox）證明了其pH響應行為。此外，還研究了水凝膠對腫瘤細胞釋放DOX的殺傷作用。體外降解和細胞毒性檢查表明水凝膠具有生物可降解性和生物相容性。因此，這種多糖基可注射自愈pH響應性水凝膠有望成為藥物遞送載體。

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編輯 | SIT Meng

來源 | 上應之研稿件

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