研究揭示人類中已有的和從頭的SARS-CoV-2體液免疫
作者：
小柯機器人發布時間：2020/11/9 22:12:02
英國弗朗西斯·克裡克研究所George Kassiotis等研究人員合作揭示了人類中已有的和從頭的SARS-CoV-2體液免疫。2020年11月6日，《科學》雜誌在線發表了這項成果。
使用SARS-CoV-2蛋白識別抗體的多種檢測方法，研究人員可以檢測到以前存在的體液免疫。通過基於流式細胞術的方法，研究人員在未感染SARS-CoV-2的個體中檢測到了SARS-CoV-2突刺糖蛋白（S）反應性抗體，並且在兒童和青少年中特別普遍。它們主要是IgG類，並靶向S2亞基。
相比之下，SARS-CoV-2感染可誘導更高滴度的SARS-CoV-2 S反應性IgG抗體，既靶向S1和S2亞基，也伴隨IgM和IgA抗體，持續整個觀察期。
值得注意的是，未感染SARS-CoV-2的供體血清對SARS-CoV-2和SARS-CoV-2 S假型表現出特定的中和活性。區分以前存在的和從頭的免疫對於人們理解SARS-CoV-2感染的易感性和自然過程至關重要。
據悉，新型冠狀病毒的傳播可能會在人類中遇到以前存在的免疫力。
附：英文原文
Title: Preexisting and de novo humoral immunity to SARS-CoV-2 in humans
Author: Kevin W. Ng, Nikhil Faulkner, Georgina H. Cornish, Annachiara Rosa, Ruth Harvey, Saira Hussain, Rachel Ulferts, Christopher Earl, Antoni G. Wrobel, Donald J. Benton, Chloe Roustan, William Bolland, Rachael Thompson, Ana Agua-Doce, Philip Hobson, Judith Heaney, Hannah Rickman, Stavroula Paraskevopoulou, Catherine F. Houlihan, Kirsty Thomson, Emilie Sanchez, Gee Yen Shin, Moira J. Spyer, Dhira Joshi, Nicola O』Reilly, Philip A. Walker, Svend Kjaer, Andrew Riddell, Catherine Moore, Bethany R. Jebson, Meredyth Wilkinson, Lucy R. Marshall, Elizabeth C. Rosser, Anna Radziszewska, Hannah Peckham, Coziana Ciurtin, Lucy R. Wedderburn, Rupert Beale, Charles Swanton, Sonia Gandhi, Brigitta Stockinger, John McCauley, Steve J. Gamblin, Laura E. McCoy, Peter Cherepanov, Eleni Nastouli
Issue&Volume: 2020/11/06
Abstract: AbstractZoonotic introduction of novel coronaviruses may encounter preexisting immunity in humans. Using diverse assays for antibodies recognizing SARS-CoV-2 proteins, we detect preexisting humoral immunity. SARS-CoV-2 spike glycoprotein (S)-reactive antibodies were detectable by a flow cytometry-based method in SARS-CoV-2-uninfected individuals and were particularly prevalent in children and adolescents. They were predominantly of the IgG class and targeted the S2 subunit. By contrast, SARS-CoV-2 infection induced higher titers of SARS-CoV-2 S-reactive IgG antibodies, targeting both the S1 and S2 subunits, and concomitant IgM and IgA antibodies, lasting throughout the observation period. Notably, SARS-CoV-2-uninfected donor sera exhibited specific neutralizing activity against SARS-CoV-2 and SARS-CoV-2 S pseudotypes. Distinguishing preexisting and de novo immunity will be critical for our understanding of susceptibility to and the natural course of SARS-CoV-2 infection.
DOI: 10.1126/science.abe1107
Source: https://science.sciencemag.org/content/early/2020/11/05/science.abe1107
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